Objective To evaluate the diagnostic value of combined detection of ischemia modified albumin(IMA) and high-sensitivity troponin I(hs-TnI) in acute coronary syndrome(ACS) by using ROC curve.Methods 31cases of acute coronary syndrome(ACS), 31cases of non-ischemic chest pain group which had been confirmed by coronary angiography, and 31cases of healthy controls were involved in this study. The serum levels of IMA, hs-TnI, Myo, TnI and CKMB were measured,and the Logistic regression model and the ROC curves were used to evaluate the diagnostic value for ACS. Results The AUC of ROC curve for IMA and hs-TnI were 0.840 and 0.945 respectively. The AUC of the “IMA + hs-TnI” combination for ACS was 0.967. The diagnostic sensitivity and specificity was 90.3% and 93.5%, respectively, which was higher than that any of the biomarkers alone and the combination of “TnI+ Myo+ CKMB”. Conclusion IMA and hs-TnI both have certain value for diagnosing ACS. The combination of IMA and hs-TnI plays a better role in diagnosing ACS than that either IMA, hs-TnI alone or the “TnI+ Myo+ CKMB” combination.