Objective To explore the influence of storage temperature and time on blood cell examination of vein blood by using EDTA-K₂ anticoagulant. Methods Total 91blood samples were divided into tree groups and stored in different temperature and time. The blood cells were examined by using XT-1800i automated hematology analyzer. 31blood samples were stored in 4℃ and analyzed at 0.5, 24, 48 and 72 h; 30 blood samples stored at 20℃ and analyzed at 0.5, 2, 4, 8, 24h;and 30 blood samples stored at 20℃ and analyzed at 24h, 48 and 72h. The results at 0.5 h were chosen as controls. Results Compared with controls, there was no substantial change in WBC, RBC, Hb, MCH and WBC differentials at 20℃ up to 24 h (P＞0.05), but other parameters showed statistically significant difference (P＜0.05). There was substantial change in WBC, RBC and Hb up to 72h at 20℃ (P＜0.05), and other parameters showed significant difference after 24h (P＜0.05). At 4℃, the WBC, RBC, MHC and Hb were stable for up to 72h, and the HCT, PLT and WBC differentials were stable for 48h, and other parameters showed significant changes after 24h. Conclusion The examination results of blood cells were more stable when the blood sample stored at 4℃, but the changes of individual parameters should be considered in order to yield more reliable results.