Objective To investigate the expression of mouse Cpne5 gene mRNA in various tissues and its location in embryo. Methods After total RNA of main organs extracted, Cpne5 mRNA were detected by RT-PCR. The probe targeting Cpne5 mRNA was synthesized, and the Cpne5 mRNA expression of different gestational age mice embryos were detected by whole-mount hybridization in situ.Results Cpne5 mRNA was detected in ten kinds of tissues of adult mouse. High expression of Cpne5 mRNA was detected in cerebrum, cerebellum, testis and lung, but no signal in liver and muscle. In neonatal mice, the highest expression of Cpne5 mRNA was found in brains, followed by the tissues of eyes and kidney. The expression level in lung and liver were lower. The concentration of antisense probes transcripted by SP6 promoter was 100ng/μL, and the concentration of sense probes transcripted by T7 promoter was 10ng/μL. The hybridization experiments showed that Cpne5 mRNA was mainly expressed in telencephalon, diencephalon, mesencephalon and rhombomere of mouse embryo. Conclusion The mouse Cpne5 mRNA are highly expressed in brain tissues of neonatal and adult mice and located in brain in the process of mouse embryonic development.
JIN Yan-bin
,
DING Xue-feng
,
SI Hui-yuan
,
WU Yan-rui
,
WANG Xiao-wen
,
WU Yan
,
FAN Wen-hong
. Expression of Mouse Cpne5 Gene mRNA in Various Tissues and Its Location in Embryo[J]. Labeled Immunoassays and Clinical Medicine, 2013
, 20(2)
: 93
-95
.
DOI: 10.11748/bjmy.issn.1006-1703.2013.02.011
[1] Tomsig J L,Creutz C E. Copines:a ubiquitous family of Ca2+-dependent phospholipid-binding proteins [J]. Cell Mol Life Sci, 2002,59(9):1467-1477.
[2] Tomsig J L, Sohma H, Creutz C E. Calcium-dependent regulation of tumor necrosis factor-α receptor signaling by copine[J].Biochem. J, 2004, 378:1089-1094.
[3] Li Y, Gou M, Sun Q, et al. Requirement of calcium binding, myristoylation, and protein-protein interaction for the Copine BON1 function in Arabidopsis[J].J Biol Chem,2010,285:29884-29891.
[4] Ramsey C S, Yeung F, Stoddard P B,et al. Copine-I represses NF-kappa B transcription by endoproteolysis of p65[J]. Oncogene,2008,27(25): 3516-3526.
[5] Heinrich C, Keller C, Boulay A, et al. Copine-III interacts with ErbB2 and promotes tumor cell migration[J]. Oncogene,2010,29(11):1598-1610.
[6]唐元家,邹洪,谢青莲,等. N-copine基因反义核酸对原代培养的小鼠皮质神经元的影响[J].中国病理生理杂志,2006,22 (1):80-83.
[7] Aquilina A, Korda M, Bergelson J M, et al. A novel gain-of-function mutation of the integrin α2 VWFA domain[J]. Eur J Biochem, 2002,269(4):1136-1144.
[8] Lopez-Nicolas R, Lopez-Andreo M J, Marín-Vicente C, et al. Molecular mechanisms of PKCα localization and activation by arachidonic Acid,The C2 Domain also Plays a Role[J]. J Mol Biol,2006, 357, 1105-1120.
[9] 王晓文,靳雁斌,吴燕,等. CopineV蛋白的亚细胞定位[J].生物技术通讯,2006,17(2):149-151.
[10] 吴彦瑞,王晓文,靳雁斌,等. CPNE5对NF-κB转录调控活性的影响[J].军事医学科学院院刊,2009,33(3):237-240.
