Objective To investigate the characteristics of immunophenotype in patients with myelodysplastic syndrome (MDS) using the samples of peripheral blood (PB). Methods The antigen expression of myeloblasts, granulocytes and monocytes in samples of peripheral blood from 38 patients with MDS and 24 patients with Non-MDS were detected by multicolor flow cytometry with four color and CD45/SSC gating. Results The abnormal immunophenotypes were existed in myeloblasts, granulocytes and monocytes in samples of PB from MDS group. The results were showed that 16 patients (42.1%) have clustered distribution of CD34+ cells on PB myeloblasts in MDS group and 3 patients (12.5%) in Non-MDS group (P＜0.05). The abnormal expression of CD11b+ cells on PB myeloblasts in MDS group (26.3%) was higher than that of in Non-MDS group (2.6%) (P＜0.05). The numbers of SSC and the Geometric mean fluorescent intensity (G-MFI) for CD10 on granulocytes in MDS group (7.4±2.8, 4.0±3.4) were significantly lower than that of in Non-MDS (9.5±2.7, 8.2±6.6) (P＜0.01) respectively. Conclusioren The abnormal Immunophenotypes can be found in patients with MDS using PB. It may be helpful to distinguish MDS from Non-MDS.
SUN Xue-jing
,
XU Juan
,
WANG Sui-gui
,
LIU Cong-yan
,
HE Jing-juan
,
LIU Yan
,
ZHANG Wei
. Analysis of Immunophenotype of Peripheral Blood by Multi-colorFlow Cytometry in Myelodysplastic Syndrome[J]. Labeled Immunoassays and Clinical Medicine, 2013
, 20(2)
: 89
-92
.
DOI: 10.11748/bjmy.issn.1006-1703.2013.02.010
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