Objective To compare the serum levels and abnormal rate of CK and its CK-MB in the normal control and patients with seven kinds of cancer.Methods The serum levels of CK and CK-MB were detected in 108 prostate cancer patients, 139 gastric cancer patients, 101breast cancer patients, 160 pancreatic cancer patients, 101colon cancer patients, 71lung adenocarcinoma patients, 72 cervical cancer patients, and 200 healthy controls. Results Compared with the healthy control, the serum levels of CK in prostate cancer patients showed significantly increased (P＜0.05), while there was no significant difference in patients with other six kinds of cancer. Compared with the healthy control, the serum levels of CK-MB in patients with seven kinds of cancer showed no significantly difference. The abnormal rates of serum CK-MB in patients with seven kinds of cancer were significantly higher than that the healthy controls; the highest abnormal rate was in gastric cancer. Conclusion The serum levels of CK in patients with prostate cancer were significantly increased. The abnormal rates of CK-MB in gastric cancer and breast cancer group were significantly higher.