Objective To establish a stable and effective system of mouse embryonic fibroblasts (MEFs) feeder layer in order to culture mouse embryonic stem cells(ESC)in vitro.Methods Embryonic fibroblast cells for primary culture were derived from mouse embryos (pregnant 13-15 days) through trypsin digestion. Results After being treated with mitomycin C (10μg/mL), MEFs proliferation could be efficiently repressed and be made into the feeder layer for ESC Undifferentiated growth. Conclusion MEFs feeder layer could effectively support mouse embryonic stem cells to grow.
YE Jue
,
ZHANG Yan-wan
,
SHI Na
,
YANG Zi-he
,
SONG Li
,
MENG Xian-min
,
XU Rui-xia
. Preparation of Feeder Layer and Mouse Embryonic Fibroblast Cells in Primary Culture[J]. Labeled Immunoassays and Clinical Medicine, 2013
, 20(1)
: 46
-48
.
DOI: 10.11748/bjmy.issn.1006-1703.2013.01.014
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