Objective To investigate the radiolabelling method of 99Tcm-mAb109 against breast cancer, and evaluate its biodistribution and imaging property on tumor-bearing animal model. Methods Monoclonal antibody mAb109 was reduced by 2-mercaptoethanol, the concentration of reduced antibody and the number of sulfhydroxyl group on reduced antibody were determined. The radiolabelling of mAb109 with 99Tcm was carried out by using transchelating method through glucohepatonate. The labeling yield and stability of radiolabelled mAb109 were measured. The biodistribution and imaging study of 99Tcm-mAb109 were carried out on a breast tumor-bearing animal model. Results The reduced antibody mAb109 remained high integrity. The average of 5.38 sulfhydroxyl groups per reduced antibody was detected. The radiolabelling yield of 99Tcm-mAb190 was more than 90%, and 99Tcm-mAb190 was very stable in vitro. Radiolabelled antibody significantly accumulated on tumor sites and remained for long time. Tumor-bearing animal model showed clear imaging after injection of 99Tcm-mAb109. Conclusion The radiolabelling method of 99Tcm-mAb109 is successfully established, and 99Tcm-mAb109 shows potentially targeting characters for animal model borne glucose-related protein 78 positive breast tumors and is worthy for further study.
ZHAI Shi-zhen
,
LI Nan
,
LI Zhen-fu
,
XIE Qing
,
ZHANG Hong
,
DU Jin
,
YANG Zhi
. Radiolabelling and Biological Evaluation of 99Tcm-mAb109 Against Breast Cancer[J]. Labeled Immunoassays and Clinical Medicine, 2013
, 20(1)
: 39
-42
.
DOI: 10.11748/bjmy.issn.1006-1703.2013.01.012
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