Objective To develop a rapid and simple colloidal gold immunochromatographic ssay (GICA) for the detection of Vibrio cholerae group O1. Methods A colloidal gold immunochromatography test based on double-antibody sandwich assay for detecting Vibrio cholerae group O1was developed. Its sensitivity, specificity and stability were then evaluated. A total of 208 clinical specimens were determined with the prepared GICA, and the results were compared with those by bacterial culture. Results Typical detecting time was less than 10 minutes per sample. The positive samples produced a positive signal on immunochromatographic strip, whereas the negative one did not.The sensitivity of the test was 1×105 cfu/ml. Both the specificity and sensitivity of the prepared GICA in determination of 208 clinical specimens were 100%. Conclusion The gold-immunochromatography test appears to be a rapid, convenient, specific and sensitive test for detecting Vibrio cholerae group O1on site.
WANG Yu-jin
,
YANG Shu-hao
,
LIU Li
,
PANG Xiang-yu
,
SUN Chen-yang
,
LI Shan-shan
. Development of Gold-immunochromatography Test forRapid Detection of Vibrio Cholerae Group O1[J]. Labeled Immunoassays and Clinical Medicine, 2013
, 20(1)
: 36
-38
.
DOI: 10.11748/bjmy.issn.1006-1703.2013.01.011
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