Objective To explore the cholinergic-inducing effects of BMP4 for rat’s cerebral neural stem cells in vivo and in vitro. Methods Neural stem cells (NSC) which were isolated from two month’s old rat’s brain region were cultivated in a medium containing EGF and bFGF and then changed to cultivate with a BMP4-added medium after 24 hours. The expression of choline acetyltransferase (ChAT) of the cultivated cytes was observed by indirect immunofluorescence test and FITC-labeled Flow Cytometry at 8 days. In order to determine oriented induction of BMP4 in NSC differentiation in the rat hippocampus dentate gyrus, ChAT immunocytochemistry was performed at 14 days after BMP4 injection. Results After cultivated with BMP4-added medium, about 34% cells showed neuron-like character observed by microscope, and more positive cells were found in the experimental group cells. The fluorescence intensity was stronger, while less positive cells were found in the control group. The results of differentiational efficiency showed that about 16% cells in experimental group appeared ChAT-positive, while control group only 7%. The in vivo experiment showed that BMP4 injection into the rat hippocampus dentate gyrus increased cholinergic expression in the hippocampus. Conclusion BMP4 could induce cholinergic differentiation of neural stem cells in vitro and increase the number of cholinergic neurons in the adult rat hippocampus dentate gyrus.
CHANG Yan
,
YANG Wen
,
YU Xiao-hong
,
PAN Jing-kun
,
TIAN Lei
,
XUE Yi-long
. The Cholinergic-inducing Effects of BMP4for Rat’s Cerebral Neural Stem Cells[J]. Labeled Immunoassays and Clinical Medicine, 2013
, 20(1)
: 25
-30
.
DOI: 10.11748/bjmy.issn.1006-1703.2013.01.009
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