Abstract Objective To investigate the clinical distribution and drug sensitivity of Pathogens isolated from Patients with liver cirrhosis complicated with spontaneous bacteria peritonitis. Methods Clinical distribution and drug sensitivity of bacterial strains isolated from ascites samples of patients with liver cirrhosis complicated with ascites symptoms were retrospectively analyzed. Results The positive strains in 638 samples of patients with liver cirrhosis complicated with spontaneous bacteria peritonitis were 67 (10.50％). Total 39 bacteria strains were cultured from these positive strains and there were 19 gram negative bacilli and 20 gram positive cocci. The isolation rates of mainly clinical strains ranged from high to low were 17.9% for enterococcus, 17.9% for streptococcus, 15.4% for Staphylococcus aureus, 15.4% for Escherichiacoli, 7.7% for Pseudomonas aeruginosa, and 25.7% for others. The cultured Gram-negative bacilli were most sensitive to Carbon penicilliumene and aminoglycoside with 83%, and the cultured Gram-positive bacteria were sensitive to vancomycin about 100%. Conclusion Escherichiacoli in gram negative bacilli and Ecfaecium in Gram positive cocci might be the main Pathogens causing liver cirrhosis complicated with SBP. It’s necessary to timely detect the pathogens for better clinical treatments to patients with liver cirrhosis complicated with ascites symptoms.