Abstract: Objective To investigate the association of single nucleotide polymorphisms (SNP) in complement receptor type 1 (CR1) gene and the level of CR1 molecules on erythrocytes in healthy people. Methods The five tag SNPs in CR1 gene from 215 cases of healthy people were detected with multiplex PCR-fluorescent labeled single base extension-tag microarray hybridization genotyping technique, and the level of CR1 molecules on erythrocytes from 81 cases were measured by flow cytometry. The Hardy-Weinberg equilibrium test for genotypic frequencies of 5 tag SNPs was carried out with the Arlequin 3.11 software, and the SPSS V13.0 software was used for comparison of inter-group differences of CR1 level, and genotypic and allelic frequencies, and also for the analysis of single and multiple factors which were correlated with the level of CR1 on erythrocytes. Results There were no statistical significance in genotypic and allelic frequencies of SNPs in CR1 gene between healthy male and female (P>0.05), and the geometric mean fluorescence intensity ratio of CR1 (CR1-GMFIR) was 3.36±1.26. The CR1 level of erythrocytes were different among groups divided by genotypes of rs11118167T>C and rs9429945C>T (P <0.01), and the CR1 level of carrier of TC and CC genotypes of rs11118167T>C were both lower than that of TT genotype, while the CR1 level of carrier of CT and TT genotypes of rs9429945C>T were both lower than that of CC genotype (P <0.01). Among age, gender and genotypes of 5 tag SNPs, CR1-GMFIR were negatively correlated with rs4844600G>A (GG/GA/AA), rs9429945C>T (CC/CT/TT) and rs11118167T>C (TT/TC/CC) (P <0.05). The rs11118167T>C, followed with rs9429945C>T were the major factors which influenced CR1-GMFIR. Conclusion The rs11118167T>C and rs9429945C>T are the major influencing factors of the CR1 molecule level of erythrocytes.