Abstract: Objective To evaluate the performance of Sysmex UF-1000i urine sediment analyzer in order to meet the requirement of the ISO15189 laboratory accreditation. Methods A series of tests with UF-1000i sedimentary analyzer of precision，accuracy，carryover and linear range were detected，as well as the biological reference interval was verified．Results Precision, accuracy, carryover and reportable range of UF-1000i were all in the allowable range supplied by factory authorities and the results of biological reference interval test met the requirements. Conclusion The performance of the Sysmex UF-1000i Urine Sedimentary analyzer was satisfied, it could be used for screening analysis of urine sedimentary.