Abstract：Objective To investigate the viability and migration of lung cancer NCI-H147 cells after IL-8 stimulation, and further study the molecular mechanism. Methods The NCI-H157 cells was cultured in vivo, and it was stimulated by different concentrations of IL-8. The cell viability was detected by MTT assays and the migration ability was examined by using the wound-healing and the Transwell assays, respectively．The expression of Rac 1 and Cdc 42 were both detected by Western blot assay. Results The MTT assay showed that the viability of NCI-H147 was increased with different concentrations. Both the Wound-healing and the Transwell assays indicated the migration of NCI-H157 was enhanced dramatically with a dose-dependent manner. The Western blot assays indicated that the expression of Rac1 and Cdc42 were up-regulated with increasing the concentrations of IL-8, and the change of Cdc42 showed more apparently. Conclusion The cell viability and migration were increased by IL-8, which may be associated with the expression of Rac1 and Cdc42.