Abstract: Objective To explore the diagnostic value of serum miR-21, miR-221, miR-222 and miR-10b in patients with glioma. Methods The relative expression of serum miR-21, miR-221, miR-222 and miR-10b in 34 cases of low-grade glioma patients and 50 high-grade glioma patients were detected by Real-time PCR. The diagnostic value of these serum markers were analyzed by using receiver operating characteristic (ROC) curve. Results Compared to low-grade glioma group, the relative expressions of serum miR-10b, miR-21 and miR-221 in high-grade glioma showed significantly increased, but the relative expressions of serum miR-222 showed no significantly difference. The AUC of miR-10b in ROC curve was the highest (0.722) and its sensitivity and specificity for the diagnosis of low-grade glioma were 79.41% and 78.00% respectively. Binary Logistic regression analysis was used to evaluate the joint diagnostic value of serum miR-10b, miR-21 and miR-221 for glioma, its AUC was 0.915 and the sensitivity and specificity were 85.29% and 88.00%, respectively. Compared to the AUC of miR-10b, miR-21 and miR-221, the AUC of joint diagnosis showed significantly increased (P = 0.026, P = 0.012, P = 0.008). Conclusion Joint diagnosis of miR-10b, miR-21 and miR-221 may provide a potential method for the diagnosis of the patients with glioma.