Abstract：Objective To investigate correlation between hepatitis B virus genome viral load and PreS1Ag in pregnancy women. Methods 558 cases of pregnancy women were involved in this study, and the levels of HBV DNA were detected by PCR technique and PreS1Ag were detected by enzyme linked immunoassay. Results The PreS1Ag positive rate was 88.6% in 221 HBV DNA positive cases and the PreS1Ag positive rate was 56.9% in 337 HBV DNA negative cases (P<0.05). The positive rate of PreS1Ag was not increased with the HBV DNA increasing in the HBV DNA positive samples. There was correlation between positive rate of HBV DNA and PreS1Ag, but no correlation between hepatitis B virus genome viral load and PreS1Ag. There is correlation between positive rate of HBV DNA and PreS1Ag in the samples which the model was HBsAg, HBeAg and HBc positive. There was no correlation between positive rate of HBV DNA and PreS1Ag in the samples which the model was HBsAg, anti-HBe and anti-HBc positive. Conclusion The results in this study suggest that HBV DNA test are more important in the pregnancy women who have HBsAg, anti-HBe and anti-HBc positive.