Abstract: Objective To determine the distribution and drug resistance of pathogen in patients with ophthalmic infection, and to provide evidences for the clinical usage of antibacterial agents. Methods Drug sensitive were studied by Kirby-Bauer methods, and the date of MIC were analyzed by WHONET5.6. Results Coagulate-negative staphylococci (CNS) accounted for the most of all the pathogen identified from infectious patients, followed by Escherichia coli. The resistance rate of CNS to levofloxacin and gentamycin were lower than that of β-lactams and Macrolides. The resistance rate of Escherichia coli to the most antimicrobial agents was low, including amikacin and ciprofloxacin. Conclusion The primary pathogens of patients with ophthalmic infection are CNS and Escherichia coli. Both organism display low drug resistance rate to quinolones and aminoglycoside, and can be used as the drug of choice for the treatment of ophthalmic infection.