Abstract: Objective To investigate the bacterial distribution characteristic and antibiotic resistance of pathogens isolated from cervical secretion of premature rupture of the membrane cases. Methods 572 cases of pregnant women with premature rupture of membranes in our hospital were retrospectively analyzed by the results of bacterial culture and drug resistance of cervical secretion. Results A total of 212 pathogenic bacteria strains were found positive among 572 cervical secretion, accounting for 37.1%. Among 212 strains of pathogens, gram-positive bacteria accounted for 62.3% (132 strains), Staphylococcus epidermidis and Staphylococcus aureus were the main bacteria, and there were no resistant strains to vancomycin, linezolid and teicoplanin. Gram negative bacteria accounted for 26.4% (56 strains). Escherichia coli was the main bacteria, and showed low resistance to the piperacillin / tazobactam, imipenem, fourth generation cephalosporin (cefepime), Amikacin, aztreonam and levofloxacin. Fungi accounted for 11.3% (24 strains). Candida albicans was the main bacteria. Conclusion For the prevention and treatment of premature rupture of membranes, rational use of antibiotics should be based on the results of bacterial culture and resistance.