Abstract: Objective To explore the value of joint detection of four kinds of tumor markers in the diagnosis of lung cancer. Methods 90 cases who had been diagnosis as lung cancer were selected as observation group. 80 cases with benign pulmonary disease were selected as benign groups. The serum SCC, CEA, CYFRA21-1 and NSE levels in all cases were detected. Results The serum levels of SCC, CEA, CYFRA21-1 and NSE in lung cancer group were significantly higher than that of benign lung disease group (P<0.05). The positive rate of joint detection of four kinds of tumor markers was significantly higher than that of any single detection (P<0.05). Conclusion The single detection of serum SCC, CEA, CYFRA21-1 and NSE levels in diagnosis of lung cancer has certain limitations with low sensitivity, and the combined detection could increase the sensitivity and has important reference value in the diagnosis lung cancer.