Abstract: Objective To analyze the clinical value of tuberculosis antibody detection combined with C-reactive protein (CRP) test in the determination of HIV infection combine pulmonary tuberculosis. Methods The serum CRP and tuberculosis antibody binding test in 46 cases of HIV and pulmonary tuberculosis infected patients and 40 cases of HIV infection patients with mycobacterial were determined by ELISA and immunoturbidimetric assay. The receiver operating characteristic curve (ROC) was used to determine the CRP diagnostic cutoff point. Results The detection sensitivity of tuberculosis antibody in the determination of HIV infection combine pulmonary tuberculosis was 52.2% (24/46) and with a specificity of 57.5% (23/40). The serum CRP critical point was 4.91 mg / L, sensitivity was 78.3% (36/46) with a specificity of 57.5% (23/40). The sensitivity and specificity of series combined detection were 43.5% (20/46) and 70.0% (28/40), and the parallel detection sensitivity and specificity were 87.0% (40/46) and 67.5% (27/40). Conclusion The combined tuberculosis antibody detection with CRP could have better effects with clinical value on determination of the HIV combine pulmonary tuberculosis infection.