Abstract: Objective To investigate the correlations between insulin resistance level with cortisol, aldosterone and INS-Ab in patients with various glucose tolerance levels. Method Total 115 patients with increased glucose tolerance were selected as subjects and divided into three groups based on diagnostic criteria for diabetes mellitus (DM), T2DM group (39 cases), pre-diabetes (PD) group (35 cases), and impaired glucose regulation (IGR) group (41 cases). All the subjects were carried out BMI measurement, 75g OGTT and insulin release test under fasting conditions and HOMA-IR were calculated. The levels of plasma cortisol, aldosterone and INS-Ab under fasting conditions were detected and the results were analyzed. Results There was significantly differences on HOMA-IR between T2DM, PD and IGR group with that of normal glucose tolerance group (P<0.01). The plasma cortisol was positive correlated with HOMA-IR (r=0.14, P<0.05), while INS-Ab and plasma aldosterone had no correlation with HOMA-IR. Conclusion Patients with increased glucose tolerance experience insulin resistance. The plasma cortisol may be correlated with insulin resistance in patients with concomitant obesity.