Abstract: Objective To discuss the infection status of chlamydia trachomatis (CT), ureaplasma urealyticum (UU), neisseria gonorrhoeae (NG) and human papillomavirus (HPV) infections in sexually transmitted disease (STD) cases. Methods The CT, UU, NG and HPV levels of STD male and female patients were detected by fluorescence quantitative polymerase chain reaction (PCR) combined with Taqman technology. Results The infection rates of CT, UU, NG and HPV were 7.49%, 42.92%, 3.11% and 3.06% respectively. The HPV infection showed a obvious gender differences. The UU positive rate was the highest and that in woman was significantly higher than that of in man. The super infection rate was 5.76%, the triple infection rate was 0.83%, and no fourfold infection. The CT and UU super infection was most commonly cases. The results showed infection rate in ages below 40 years old was the highest. Conclusion It is important to incorporate HPV to the detection of urogenital infections. With obvious gender differences in addition to HPV whether single and mixed infection, and it is consistent with the distribution of both infections. The age below 40 years group is the main source of infection and the high risk population, and should be considered as focus on sexually transmitted disease prevention. The super and triple infection are common, no fourfold infection, and people should pay more attention.