Objective To explore the relationships between FT4 and TT4 with TBA and ADA in patients with moderate chronic hepatitis B. Methods The serum levels of FT4, TT4, TBA and ADA in 30 patients with mild chronic hepatitis B, 34 patients with moderate chronic hepatitis B, 30 patients with severe chronic hepatitis B and 30 cases of healthy controls were detected and results were statistically analyzed. Results The serum levels of FT4, TT4, TBA and ADA in patients with moderate chronic hepatitis B were higher than that of patients with mild chronic hepatitis B and health controls (P <0.001). The FT4 levels in patients with moderate chronic hepatitis B was positively correlated with ADA and TBA levels (r = 0.412, 0.412; P = 0.016, 0.035). The TT4 level was positively related with TBA level (r = 0.387, P = 0.387), and no correlation with ADA (r = 0.124, P = 0.124). Conclusion The serum levels of FT4, TT4, TBA and ADA have important clinical significance in the evaluation of liver dysfunction in patients with moderate chronic hepatitis B.