Abstract: Objective To establish and verify the industry standard for Thyroid-Stimulating Hormone (TSH) quantitative immunoassay kit. Methods Three types of TSH immunoassay kits of including enzyme, chemiluminescent and time resolved fluorescence labeled immunoassay were used. The appearance and physical examination, the minimum detectable amount, accuracy, linearity, precision, value of quality control, specificity, and stability of immunoassay kits were verified in accordance with industry standard. Results Except the enzyme kit couldn’t meet the demand of linearity range, the others kits could meet all requirements. Conclusion The establishing of the industry standard for Thyroid-Stimulating Hormone quantitative immunoassay kit was reasonable and operable, which would help contribute to the unity Thyroid-Stimulating Hormone quantitative immunoassay kit’s quality, and offer the basis for the administration of its production, examination, circulation, clinical application and other areas.