Abstract：Objective To evaluate the performances of three different kinds of sperm analyzer including SCA from Spain, Bei Ang from Shanghai and Wei Li from Beijing. Methods The density and vitality of sperm species were detected with three analyzers and microscope, and the repeatability and linearity of different sperm analyzers were analyzed. Results The repeatability of three instruments was good with all the coefficient of variation less than 15%. The detection results with each sperm analyzer and microscope were closed (P<0.001). Conclusion It is necessary to establish the corresponding reference value range for different sperm analyzers.