Abstract: Objective To investigate HPV infection status of women in Hubei province in order to provide the basis for the prevention and treatment of the cervical cancer. Methods The infection status of HPV in 3956 patients of gynecology department was examined with gene chip technique in Hubei Zhongshan hospital from 2013 January to 2014 November. Results Among the 3956 patients，818 patients were infected with HPV (20.68％), and 624 patients were infected with HR-HPV (high risk HPV, 15.77％). Out of the 23 subtypes of HPV, HPV-16 (3.72％, 147/3956) was the most common of all types, followed by subtypes HPV-52 (3.67%, 145/3956), HPV-43 (3.64%, 144/3956), HPV-58(2.78%, 110/3956), HPV-18 (1.49%, 59/3956), HPV-33(1.06%, 42/3956), and no HPV-MM4. In the HPV positive patients, 142 (17.36％, 142/818) patients were compound infected, of whom 121 (85.21％, 121/142) patients were super-infected. Conclusion The more common high-risk HPV subtype are HPVl6, HPV52, HPV58, HPV18 and HPV33 and low-risk HPV subtype is HPV43 in Hubei province.