Objective To investigate the effect of E-selectin on genital chlamydia trachomatis infection. Methods 64 cases of mice infected by chlamydia trachomatis were divided randomly into two groups: the experiment group and the control group. Mice in the control group were treated with saline, and those in the experiment group with artificial synthesized E-selectin. At 1, 2, 3 and 4 weeks after intervention, the chlamydia trachomatis infection rate and the chlamydial shedding were calculated. Results Mice model of genital chlamydia trachomatis infection was successfully made. The chlamydia trachomatis infection rate at 1 and 2 weeks in the experiment group were higher (90.2%, 85.3%) than those in the control group (77.4%, 70.1%). The chlamydial shedding were lower in the experiment group (2.458±0.225, 1.198±0.177) than those in the control group (3.210±0.315，1.809±0.203). There was statistically significant difference. between the two groups There were not any significant differences among two groups at 3 weeks after intervention in the term of the infection rate (34% vs 24%) and chlamydial shedding (0.912±0.134 vs 1.201±0.189). There was no infection and chlamydial shedding in mice of two groups at 4 weeks after intervention. Conclusion E-selectin can facilitate the genital track infection from chlamydia trachomatis, which could provide the foundation for further illuminating the pathogenesis of genital chlamydia trachomatis infection.