Abstract: Objective To introduce an effective method to discover and eliminate the crossover contamination on the automatic biochemistry analyzer. Method Thirty-six clinical biochemistry items were tested and analyzed to screen the crossover contamination by Hitachi 7600 automatic biochemistry analyzer. After the confirmation on the existence of crossover contamination, water washing, acid washing, alkaline washing and adjustment of tests sequencing were applied to eliminate or reduce the crossover contamination. Results Thirty-nine pair tests with crossover contamination were found in this study. After water washing, acid washing and alkaline washing, twenty-six pair tests (66.7 %) with crossover contamination were improved. But the remaining 13 pair tests with crossover contamination were not improved. Then tests sequencing were adjusted, the remaining 13 pair tests with c crossover contamination were improved. Conclusion The crossover contamination is one of the major interferences to the accuracy of the biochemistry tests. However, this kind of contamination was easily to be neglected if only depending on the empirical observing. The screening, confirmation and corresponding measures should be applied to enhance the accuracy of results and provide better service to clinics when the clinical biochemistry lab with opening reagents begin to use the new automated biochemistry analyzer or change the reagents.