Objective To find out mastery of knowledge of safe blood transfusion for surgical nurses with low-seniority, so as to provide the basis for taking measures to improve the level of knowledge about safety of blood transfusion for nurses. Methods 160 nurses with low-seniority were investigated by questionnaire. Results Educational background had no impact on the cognition of safe blood transfusion, but there were differences among departments. Conclusion With the development of blood products and technology, surgical nurse managers should pay attention on the master of the knowledge about safe blood transfusion for nurses with low-seniority.