Objective To explore the diagnostic value and efficiency evaluation of whole body bone scintigraphy, tumor markers and alkaline phosphatase level in breast cancer patients with bone metastases. Methods 100 patients were involved in this retrospective analysis, 62 of them were diagnosed with bone metastases. The results of whole body bone scintigraphy, CT scan, tumor makers and alkaline phosphatase were analyzed. Results The sensitivity, specificity and accuracy of whole body bone scintigraphy and computed tomography were 88.71%, 78.95%, 85.00% and 72.58%, 92.11%, 80.00%, respectively. The concentrations of CA15-3, CEA and ALP in the patients with bone metastases were statistically higher than those patients without metastases (P<0.05). The level of CA15-3, CEA and ALP level were increased and positively correlated with the number of bone metastases (r=0.65，0.40，0.63). After treatment, the levels of CA15-3, CEA and ALP were decreased and kept consistent with the number of bone metastases in patients with bone metastases. Conclusion The whole body bone scintigraphy shows high sensitivity, specificity and accuracy in the diagnosis of bone metastases, and the detection of CA15-3, CEA and ALP have important value in the evaluation treatment efficacy of breast cancer patients with bone metastasis.