Abstract：Objective To analyze the distribution and drug resistance of pathogens causing intravascular catheter-related bloodstream infections (CRBSI), and provide doctors with laboratory evidence of CRBSI diagnosis. Methods A retrospective analysis of 107 strains of CRBSI pathogens’ distributions from 91 inpatients whose catheter culturing was positive. Results There were 91 (1.4%) patients diagnosed as CRBSI among 6688 cases. They were from ICU, Burns Branch, Department of Nephrology and Emergency Room. A total of 107 strains of pathogens, gram-positive bacteria accounted for 28% (30 strains), 12.1% (13 strains) of which were coagulase-negative Staphylococcus, 10.2% (11strains) of which were Staphylococcus aureus, the susceptibility rates to vancomycin, linezolid and tigecycline of Staphylococcus arrived at 100%, non-fermenting gram-negative bacteria accounted for 63.6% (68 strains). The first three by rank order of the CRBSI pathogens were Burkholderia cepacia (20.6%), Acinetobacter baumannii (12.1%), Pseudomonas aeruginosa (12.1%). The multi-drug resistant was more serious in non-fermenting bacteria, fungi accounted for 8.4% (9 strains), and in which Candida albicans were sensitive to fluconazole, itraconazole, amphotericin B and 5-fluorocytosine. However, C.glabrata and C.krusei had different degrees of intermediaries and antimicrobial drugs. Conclusion CRBSI’ major pathogens are Staphylococcus, non-fermenting Gram-negative bacteria and Candida species, attaching importance to pathogen and drug sensitivity inspection can guide controlling bacteria of CRBSI and rational using clinical antibiotics.