Abstract: Objective To investigate the characteristic of serum osteocalcin and β-CrossLaps level in gestational diabetes mellitus. Methods 30 gestational diabetic patients (GDM group) and 30 pregnant women with normal glucose tolerance (NGT group) were included in this study. The levels of serum glucose, N-MID osteocalcin and β-crosslaps were detected and compared. Results Serum N-MID osteocalcin and β-crosslaps levels in GDM group were significantly higher than those in NGT group (P=0.017 and P=0.018, respectively). Conclusion Bone resorption and turnover level in gestational diabetic patients are higher than pregnant women with normal glucose tolerance.