Abstract: Objective To explore the correlation of serum soluble ST2 (sST2) and brain natriuretic peptide (BNP) in patients with heart failure and its clinical diagnostic values. Methods 60 cases of heart failure were classified according to the New York Heart Association (NYHA) heart function standard. All patients were asked in detail about medical history and completed sST2 and BNP check on the basis of routine examination after admission. Results The serum levels of sST2 and BNP in the Observation group were higher than those in the control group (P<0.01). There were significant differences in sST2 and BNP blood concentration levels between different classification groups（P< 0.05）. The higher level of cardiac function was wit higher serum sST2 and BNP. Spearman correlation analysis showed that the serum levels of ST2 and BNP increased with the severity of heart failure. ROC curve showed that the correlation coefficients for sST2 and BNP were 0.922 and 0.881. Conclusion the serum levels of sST2 and BNP are positively related to the severity of heart failure, thus it can be used for the diagnosis of heart failure.