Objective To prepare self-made quality control material of qualitative urine HCG critical value, and establish the corresponding internal control procedures to evaluate its effect in practical application. Methods The known HCG concentration of urine was diluted to 25IU/L with the physiological saline, and then the stabilizer and preservatives were added. The solution was divided into plastic centrifuge tube and then stored at room temperature, 4 ℃ and -20 ℃. The samples were daily monitoring and continuous for one year to evaluate its stability. The urinary HCG internal quality control procedures were established in the South Huiqiao laboratory information system to evaluate the detection performance and application effect. Results The self-made quality control samples of qualitative urine HCG critical value showed good stability at room temperature, 4 ℃, -20 ℃, which could be stabilized for 121 days, 300 days, 365 days or more. Conclusion The self-made quality control material of qualitative urine HCG critical value combined with the internal quality control procedures could be used as the internal quality control of urine HCG.