Objective To explore the effect of batroxobin on mechanism of bleeding and clotting in patients with sudden deafness. Methods 60 patients with diagnosis of sudden deafness were treated with batroxobin in addition to other applications of dilate blood vessels, nerve nutrition and antiviral drugs. The levels of PT, TT, APTT, FIB, and D-Dimer in patients before and during treatment and 40 cases of healthy controls were detected. Results There were no statistically difference on the levels of PT, TT, APTT and D-Dimer between patients and healthy controls (P > 0.05), but the FIB level in patients with sudden deafness was increased compared with controls (P < 0.05). The levels of PT, TT, APTT and D-Dimer in sudden deafness patients during batroxobin were increased compared with before the treatment (P < 0.05), but the FIB levels were decreased (P < 0.05). Conclusion Batroxobin has good antithrombotic effect and might be an ideal drug in the treatment of sudden deafness.