Objective To investigate the infection of Hepatitis B in pregnant women and to provide data support for the protection of pregnant women with hepatitis B. Methods The five quantitative markers of Hepatitis B in 5739 pregnant women were detected by using TRFIA. Results The positive rate of hepatitis B surface antibody in pregnant women was 51.93%, the average positive rate of high immune response was 30.49% and the positive rate of low immune response was 21.43%. Conclusion There is a certain proportion in pregnant women infected with HBV. The susceptible, quantitative detection of hepatitis B can better reflect the body dynamic immune condition of hepatitis B infection, to prevent HBV infection and block mother to child transmission. It has important significance to improve the quality of the population.