Objective To investigate the effects of 99Tc-Methylenediphosphonate (99Tc-MDP) on serum antioxidant indexes and thymus and spleen index induced by D-galactose in aged mice. Methods Total 48 healthy female Kunming mice were randomly divided into 6 groups: normal control group, model group, vitamin E and low, middle and high dosage of 99Tc-MDP. After the last administration, the mice blood were took by removing the eye and the contents of malondialdehyde (MDA), activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-PX ) in serum were detected by biochemistry method. Meanwhile, the body weight, and the thymus and spleen of each mouse were measured. Results Compared with the model group, the thymus, spleen index in experimental treatment group were increased; the activity of SOD and GSH-PX in serum were enhanced significantly in the experimental treatment group, the serum MDA content were decreased．The administration of 99Tc-MDP and vitamin E had no different degrees in the elevating SOD and GSH-PX activities, and reduction of the content of serum MDA. Compared with the normal control group, the activity of SOD and GSH-PX in serum were reduced significantly in the model group, the MDA content of serum were increased．Conclusion 99Tc-MDP may reduce the effect of free radical damage to the body and play a role in delaying aging by increasing the immune function.
XUE Xiu-qing, ZHENG jian, SU Cheng-hai
. Effects of 99Tc-MDP on Serum Antioxidant Indexes Induced by D-galactose in Aged Mice
[J]. Labeled Immunoassays and Clinical Medicine, 2014
, 21(6)
: 718
.
DOI: 10.11748/bjmy.issn.1006-1703.2014.06.031
