Objective To investigate serum protein spectrum expression changes for patients with gastrointestinal disease before and after acupuncture. Methods Blood biochemical indexes and serum protein spectrum for 7 patients with gastrointestinal diseases before and after acupuncture were detected by 7600DDP and MALDI-TOF mass spectrometer. Results Compared with patients with gastrointestinal disease before acupuncture treatment, the triglyceride (TG) level of patients with gastrointestinal diseases after acupuncture treatment decreased. There were 2 differential protein peaks (2625 (m/z), 2742 (m/z) between patients with gastrointestinal disease before acupuncture treatment group and after acupuncture treatment group. There were 4 differential protein peaks (2767（m/z）, 2742（m/z）, 2754（m/z），2568（m/z）) between patients with gastrointestinal disease before acupuncture treatment group and the control group; we establish two-dimensional analysis with 2767 (m/z) (x axis) and 2742 (m/z) (Y axis) and found that acupuncture treatment has obvious effect on patients with gastrointestinal disease , it is close to the normal. Conclusion 2742 (m/z) and 2767 (m/z) play an important role in acupuncture treatment mechanism of patients with gastrointestinal disease.