Objective To investigate the effect of the anxiety of family members on glioma patients the stress state during perioperative period. Methods 112 glioma patients who needed operations were recruited in our study. These patients were divided into controls group, nursing intervention on family members, nursing intervention on patients, nursing intervention on family members and patients group, randomly. Each group included 28 patients. The blood pressure, heart rate, serum levels of cortisol (Cor) and C reactive protein (CRP) of patients were detected on four time points, which were the day before operation (T1), before anesthesia (T2), after surgery (T3) and 24 hours after surgery (T4). Results On the T1 time point, the blood pressure, heart rate, serum levels of Cor and CRP were no significantly different among the four groups (p>0.05). On T2 and T3 time point, the blood pressure, heart rate, serum levels of Cor and CRP in nursing intervention on both family members and patients group were all significantly lower than that of other three groups (p<0.05). On the T4 time point, the blood pressure, heart rate, serum levels of Cor and CRP were all significantly different in the four groups (p<0.05). Conclusion Nursing intervention on both family members and patients could lower the stress levels of giloma patients during perioperative period. It could be better than those nursing interventions on patients or on family members only.