Objective To analysis serological infection modules of hepatitis B virus (HBV) in north China. Methods The serum HBV markers in 16553 patients were quantitatively detected with Architect chemiluminescence immunoassay (CLIA). Results 5584 patients were never infected by HBV, composed by 33.7%. 1431 patients were HBsAg carriers, composed by 8.6%. 7 kinds of serological modules were found in the previous or current HBV infenction patients. The first, second, third were: HBsAb alone positive (3983 cases, 24.1%), HBsAb and HBeAb and HBcAb positive (2492 cases, 15.1%), HBsAb and HBcAb positive (2085 cases, 12.6%), respectively. There were 323 cases of rare modules, composed by 2.0%. Among the HBsAb positive (≥10mIU/mL) patients, there were 38.4% in which HBsAb level was lower than 100mIU/mL， and only 31.5% patients over 500mIU/mL. Conclusion The positive rate of HBV was higher in north China than that of other areas (7.18%). In the HBsAb positive patients, there were almost one third in which HBsAb level was lower than 100mIU/mL, so action should be taken to protect from HBV infection.