Objective To provide accurate and rapid laboratory information for diagnosis and treatment of group B streptococcus（GBS） infection in Pregnant Woman. Methods GBS of swabs for reproductive tract in pregnant woman was detected by rapid immunochromatographic assay and traditional culturing method. Results The rapid immunochromatographic assay was showed more advantages than that traditional culturing method and the compliance rate was 98.0%. Conclusion The rapid immunochromatographic assay is a rapid and useful method to detect GBS of reproductive tract in pregnant woman for diagnosis and treatment of GBS infection.