Objective To explore the therapeutic effect of malignant cancer treated with intervention of 90Y-lasbelled glass microspheres. Methods 18 cancer patients with primary tumor located in liver (6 cases), lung (5 cases), cervical lymph node metastases (5 cases), maxillary sinus carcinoma (1 case) and chest wall metastasis (1 case) were included in the study. All patients were confirmed by pathological diagnosis. 90Y-lasbelled glass microspheres was injected with or without the help of type-B ultrasonic and CT. Results Out of 18 cases of patients, 2 cases were CR, 9 cases were PR, 6 cases were SD and one case was PD. 14 cases were showed pain relief and the other 4 cases were no significant relief of pain. Conclusion This method is effective in the therapy of malignant cancer, and it has great potential for clinical application.