Objective To evaluate the occurrence regularity and high risk factors thrombophilia of spontaneous abortion and to provide effective precautions and treatments. Methods 1300 patients with spontaneous abortion were divided into four groups randomly according the abortion times, one, two, three and more. The thrombosis markers in these patients were detected. Results The thrombosis markers such as antithrombase Ⅲ, plasminogen activity, fibrin breakdown products, plasminogen activator inhibiting factor, tissue plasminogen activator had remarkable statistical difference to the bad pregnancy outcome (P<0.01）, it was also increased with abortion times (P<0.01）. The activity of protein C and protein S had remarkable statistical difference to the bad pregnancy outcome (P<0.01). Conclusion The activity of protein C and protein S may be the characteristic markers in the diagnosis and treatment to thrombophilia of recurrent spontaneous abortion. The antithrombin Ⅲ, activity of plasminogen, fibrin degradation product, plasminogen activator inhibiting factor are the influence factors for recurrent spontaneous abortion. These markers become more evident along with the abortion times.