Abstract: Objective To investigate the correlation between the levels of HBV-DNA measured by FQ-PCR and the markers of Hepatitis B virus detected by CLEIA. Methods The HBV-DNA positive samples which were detected by FQ-PCR were tested by CLEIA for the markers of Hepatitis B virus, namely Five markers of Hepatitis B. Results The samples which HBsAg, HBeAg and HBcAb tested positive and samples which HBsAg, HBeAb and HBcAb tested positive shared the highest proportion of the total sample, and the proportion of low, medium and high HBV-DNA load were 17.2%, 17.2%, 65.5% and 81.8%, 15.2%, 3.0%, respectively (P <0.05). The difference between the HBV-DNA load of HBeAg-positive samples and HBeAb-positive samples was statistically significant (P <0.05). Conclusion The aspects of HBV infection course reflected in HBV-DNA levels detected by FQ-PCR and in the levels of Five markers of HBV detected by CLEIA are basically in line, but the value of the quantitative results of five markers of HBV are not directly related to the value of HBV-DNA load.