Objective To evaluate the value of the thyroid autoimmune antibodies (TPOAb) in the diagnosis of Hashimoto's thyroiditis. Methods We searched CNKI, VIP Information, Wanfang data for studies on he TPOAb and Hashimoto's thyroiditis. We also reviewed reference listserv to seek the data missed by our search. Random effects meta-analysis was used to pool data. Results The level of TPOAb and the percentage of TPOAb positive in the Hashimoto's thyroiditis patients were both significantly higher than the control group. The pooled sensitivity and pooled specificity on the thyroid autoimmune antibodies (TPOAb) in the diagnosis of Hashimoto's thyroiditis were 0.89（95% CI：0.87-0.91）and 0.96（95% CI：0.95-0.97）respectively, and the SROC AUC was 0.9843. Conclusion The sensitivity and specificity on the thyroid autoimmune antibodies (TPOAb) in the diagnosis of Hashimoto's thyroiditis are both in a high level. TPOAb might be a good biomarker in diagnosis of Hashimoto's thyroiditis.