Abstract: Objective To evaluate the performance of treponema pallidum （TP） antibody detected by ELISA. Methods According to the CLSI document EP12-A2, the reproducibility of TP antibody measurement within the range of ±20% cut off by ELISA was analyzed, and the concordance was compared with TP particle agglutination assay (TPPA). Results The samples with +20% concentrations from the cut-off point yielded positive rate ≥95%. The coefficient of variation (CV) was 14.8%. The yielded negative rate was ≥95% in samples with -20% concentrations from the cut-off point. The CV was 19.6%. The comparison of the two analysis assay was showed that the concordance was 99.6%. The 95% confidence interval was 98.8%~99.6%. Conclusion The reliable results could be acquired by ELISA when it utilized in the detection of TP antibody with the concentration away from the range of   ±20% cut off. The ELISA could replace TPPA for the treponema pallidum antibody detection.