Abstract：Objective To study analytical performance of domestic serum free fatty acid (FFA) detection kits. Methods Two domestic serum free fatty acid detection kits were chosen to detect concentration of FFA in pooled serum specimens and the samples were also measured by Abbott c8000 Automatic Biochemistry Analyzer to evaluate the linearity, precision, accuracy, and interference of the two kits. Results The interassay coefficient variation (CV) of two domestic reagent were 7.6% and 5.8%, and the intraassay CV was 9.6 % ; while the interassay CV of foreign reagent were 0.7% and 2.8%, and the intraassay CV was 2.2 %. The average recovery of domestic reagent and foreign reagent were100.3% and 97.76% respectively, there was no significant difference (P>0.05). The analysis of linear range indicated that concentration of FFA within 3.11 mmol/L (domestic kits) and 3.23 mmol/L (foreign kits) had high linear correlation, and the difference had no statistical significance (P>0.05). On the condition of hemoglobin (<0.12mmol/L), bilirubin (<0.34mmol/L), triglycerides(<11.3mmol/L), Vitamin C (<1.2mmol/L), there were no significant effect on quantitative detection of FFA in 50 serum samples detected by both kits, which showed high correlation and low deviation (P>0.05). Conclusion Compare to foreign reagents, domestic reagents has relatively lower precision. They all have high consistency and correlation and no significant difference are found.