Abstract: Objective To explore the best conservative conditions of full blood samples for glycosylated hemoglobin (HbA1c) measurement by high performance liquid chromatography (HPLC). Methods 30 full blood samples were randomly divided into 2 groups, and stored at 6 conditions, which included 2 temperatures and 3 conservative times. HbA1c values were determined by using HPLC. Results It was shown by our studies that determined values of HbA1c reduced along with conservative time. Conclusion The full blood stored at 4°C is better than that of at -20°C for the conservation of HbA1c within 2 weeks.