Abstract: Objective To explore the relationship between the serum levels of folic acid and vitamin B12 with pregnancy-induced hypertension (PIH). Methods The serum levels folic acid and vitamin B12 in 25 patients with PIH, 30 normal pregnant women and 35 normal women were measured by chemiluminescent immunoassay (CLIA). Results There is no significant difference of folic acid and vitamin B12 levels between normal pregnant women group and normal women group, the serum folic acid and vitamin B12 levels in patients with PIH were significantly lower than the other two groups (P<0.05). Conclusion The levels of folic acid and vitamin B12 might be used as predictable markers of PIH, there are good correlations between the serum levels of folic acid, vitamin B12 and PIH.