Objective To evaluate the application of chemiluminescent microparticle immunoassay (CMIA) technology in quantitative detection of pm- transfusion infection indicators. Methods The pm- transfusion serum levels of hepatitis B surface antigen (HBsAg)，hepatitis C virus antibody (anti-HCV)，anti- Treponema pallidum- specific antibody (Anti-TP) and anti - humanimmunodeficiency virus antibody ( anti - HIV ) in 22，235 cases of patients were determined with chemiluminescent microparticle immunoassay ( CMIA) . The qualitative detection of all positive samples were carried out by the corresponding enzyme- linked immunosorbent assay (ELISA)，and the anti - treponema pallidum antibody ( Anti-TP) positive samples were confirmed by Treponema pallidum antibodies gelatin particle agglutination test (TPPA) . Results The number of positive cases of HB- sAg, Anti - HCV，Anti-TP and Anti- HIV in 22 ,235 cases of patients were 3028，336，684 and 63 respectively. The qualitative positive cases among these positive samples detected by ELISA were 2472, 269, 668, and 58. 603 samples in 684 cases of Anti-TP the quantitative positive samples were confirmed as positive by TPPA. Conclusion CMIA assay for quantification of pre-transfusion infection indicators could be used as early detection of infection “window period” to reduce the missing rate. It would be very helpful in the early diagnosis and treatment of patients, and self- protection of healthcare workers.