Objective To explore the diagnostic values of serum miR-128 and miR-720 relative expressions in patients with glioma. Methods The serum miR-128 and miR-720 relative expressions were detected by TaqMan MicroRNA Assays Real-time PCR in 54 low-grade glioma patients group, 57 high grade glioma patients group and 62 healthy controls group. Results Compared to the healthy control group, miR-720 relative expression in patients with low-grade glioma were showed significant difference, miR-128 and miR-720 relative expressions in the high -grade glioma group showed significant difference. Compared to the low-grade glioma group, miR- 128 and miR-720 relative expressions in the high- grade glioma were showed significant difference. The diagnostic sensitivity and specificity of miR-128 and miR-720 relative expressions for discriminating the healthy control group and the glioma group were 92. 3% and 87. 2% , respectively. The diagnostic sensitivity and specificity for discriminating the low-grade glioma group and the high -grade glioma group were 84. 3% and 88. 9%，respectively .Conclusion The combined detection of miR-128 and miR-720 relative expressions may provide an auxiliary method for the clinical diagnosis and classification of glioma.